Высокоэффективная жидкостная хроматография в частично денатурирующих

Идентификация вариации числа копий CAPN10 у тайцев с диабетом 2 типа с помощью мультиплексной ПЦР и денатурирующей высокоэффективной жидкостной хроматографии ( DHPLC ).

 Было показано, что вариации числа копий (CNV) связаны с несколькими заболеваниями. Они могут вызывать отклонение генотипов от равновесия Харди-Вайнберга (HWE) . Исследования ассоциации генетический случай-контроль в Тайланде показали, что распределение генотипов CAPN10 Indel19 отклонялось от HWE после исправления ошибки генотипирования. Таким образом, мы стремимся идентифицировать CNV в области CAPN10 Indel19.

Метод полуколичественной денатурирующей высокоэффективной жидкостной хроматографии (DHPLC) был использован для обнаружения CNV в области маркера CAPN10 Indel19 в когорте 305 пациентов с диабетом 2 типа и 250 контрольных субъектов без диабета. CNVs в области CAPN10 Indel19 был успешно обнаружен с помощью DHPLC .

После корректировки определения генотипа на основе статуса идентифицированных CNV, генотипы CAPN10 Indel19 были хорошо приспособлены для HWE (p> 0,05) . Однако мы не обнаружили связи между генотипами CNV и риском диабета 2 типа в нашей популяции. CNV в CAPN10 были идентифицированы у тайцев. Эти CNVs приводят к отклонению от HWE генотипов CAPN10 Indel19 . После исключения идентифицированных CNV из анализа, CAPN10 Indel19 был связан с диабетом 2 типа. Информация, полученная в результате нашего исследования, может быть полезна для точности генотипирования SNP, находящихся в области CNV .

 

Нуклеаза DHPLC / SURVEYOR: чувствительный, быстрый и доступный метод анализа мутаций BRCA1 и BRCA2 в семьях рака груди.

 Наследственный рак молочной железы составляет около 10% всех случаев рака молочной железы, и гены BRCA1 и BRCA2 были идентифицированы как проверенные гены предрасположенности к этой патологии. Тестирование на мутации гена BRCA обычно основано на подходе предварительного скрининга, таком как метод частичной денатурации DHPLC и прямое капиллярное секвенирование.

Однако этот подход требует много времени из-за большого размера генов BRCA1 и BRCA2. Недавно был разработан новый недорогой и экономящий время протокол DHPLC для анализа генных мутаций с использованием расщепления нуклеазой SURVEYOR® и анализа DHPLC . Подгруппа из 90 пациентов, включенных в Программу генетического консультирования Национального онкологического центра Бари (Италия), была проведена для проверки этого подхода.

Предыдущий ретроспективный анализ показал, что 9/90 пациентов (10%) были мутированы в генах BRCA1 и BRCA2, и эти данные были подтверждены настоящим подходом. Образцы ДНК были подвергнуты Landing PCR и, впоследствии, расщеплению нуклеазой SURVEYOR (®). Ампликоны BRCA1 и BRCA2 были разделены на группы в зависимости от размера ампликона, чтобы обеспечить переваривание нескольких ампликонов.

Продукт этой реакции анализировали на системе анализа высокочувствительных фрагментов трансгеномной нуклеиновой кислоты WAVE . Оператор, выполнявший метод обследования DHPLC, в то время не знал результатов секвенирования. СЮРВЕЙЕРСКИЙ (®) нуклеаза DHPLC подход был способен обнаружить все изменения с чувствительностью 95%. Кроме того, чтобы сэкономить время и реагенты, был утвержден препарат для настройки мультиампликона.

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit
DLR-MYH1-Hu-96T	DL Develop	96T	EUR 621
Should the Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit
RDR-MYH1-Hu-48Tests	Reddot Biotech	48 Tests	EUR 500

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit
RDR-MYH1-Hu-96Tests	Reddot Biotech	96 Tests	EUR 692

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit
RD-MYH1-Hu-48Tests	Reddot Biotech	48 Tests	EUR 478

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit
RD-MYH1-Hu-96Tests	Reddot Biotech	96 Tests	EUR 662

Mouse Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit
DLR-MYH1-Mu-48T	DL Develop	48T	EUR 489
Should the Mouse Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) in samples from serum, plasma, tissue homogenates or other biological fluids.

Mouse Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit
DLR-MYH1-Mu-96T	DL Develop	96T	EUR 635
Should the Mouse Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) in samples from serum, plasma, tissue homogenates or other biological fluids.

Mouse Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit
RDR-MYH1-Mu-48Tests	Reddot Biotech	48 Tests	EUR 511

Mouse Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit
RDR-MYH1-Mu-96Tests	Reddot Biotech	96 Tests	EUR 709

Mouse Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit
RD-MYH1-Mu-48Tests	Reddot Biotech	48 Tests	EUR 489

Mouse Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit
RD-MYH1-Mu-96Tests	Reddot Biotech	96 Tests	EUR 677

Human Myosin 1(MYH1) ELISA kit
E01M0413-192T	B-Gene	192 tests	EUR 1270
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Human Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Myosin 1(MYH1) ELISA kit
E01M0413-48	B-Gene	1 plate of 48 wells	EUR 520
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Human Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Myosin 1(MYH1) ELISA kit
E01M0413-96	B-Gene	1 plate of 96 wells	EUR 685
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Human Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human MYH1/ Myosin-1 ELISA Kit
E1687Hu	Sunlong	1 Kit	EUR 571

Human MYH1(Myosin-1) ELISA Kit
EH0735	FN Test	96T	EUR 524.1
Detection range: 0.156-10 ng/ml Uniprot ID: P12882 Alias: MYH1(Myosin Heavy Chain 1, Skeletal Muscle, Adult)/Myosin-1/Myosin heavy chain 1/Myosin heavy chain 2x(MyHC-2x)
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Pig MYH1/ Myosin-1 ELISA Kit
E0133Pi	Sunlong	1 Kit	EUR 717

Bovine MYH1/ Myosin-1 ELISA Kit
E0186Bo	Sunlong	1 Kit	EUR 717

Rat Myosin 1(MYH1) ELISA kit
E02M0413-192T	B-Gene	192 tests	EUR 1270
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Rat Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Myosin 1(MYH1) ELISA kit
E02M0413-48	B-Gene	1 plate of 48 wells	EUR 520
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Rat Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Myosin 1(MYH1) ELISA kit
E02M0413-96	B-Gene	1 plate of 96 wells	EUR 685
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Rat Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Myosin 1(MYH1) ELISA kit
E04M0413-192T	B-Gene	192 tests	EUR 1270
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Myosin 1(MYH1) ELISA kit
E04M0413-48	B-Gene	1 plate of 48 wells	EUR 520
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Myosin 1(MYH1) ELISA kit
E04M0413-96	B-Gene	1 plate of 96 wells	EUR 685
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Myosin 1(MYH1) ELISA kit
E03M0413-192T	B-Gene	192 tests	EUR 1270
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Mouse Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Myosin 1(MYH1) ELISA kit
E03M0413-48	B-Gene	1 plate of 48 wells	EUR 520
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Mouse Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Myosin 1(MYH1) ELISA kit
E03M0413-96	B-Gene	1 plate of 96 wells	EUR 685
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Mouse Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Myosin 1(MYH1) ELISA kit
E06M0413-192T	B-Gene	192 tests	EUR 1270
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Goat Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Myosin 1(MYH1) ELISA kit
E06M0413-48	B-Gene	1 plate of 48 wells	EUR 520
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Goat Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Myosin 1(MYH1) ELISA kit
E06M0413-96	B-Gene	1 plate of 96 wells	EUR 685
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Goat Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Myosin 1(MYH1) ELISA kit
E08M0413-192T	B-Gene	192 tests	EUR 1270
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Canine Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Myosin 1(MYH1) ELISA kit
E08M0413-48	B-Gene	1 plate of 48 wells	EUR 520
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Canine Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Myosin 1(MYH1) ELISA kit
E08M0413-96	B-Gene	1 plate of 96 wells	EUR 685
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Canine Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Myosin 1(MYH1) ELISA kit
E07M0413-192T	B-Gene	192 tests	EUR 1270
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Porcine Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Myosin 1(MYH1) ELISA kit
E07M0413-48	B-Gene	1 plate of 48 wells	EUR 520
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Porcine Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Myosin 1(MYH1) ELISA kit
E07M0413-96	B-Gene	1 plate of 96 wells	EUR 685
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Porcine Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Myosin 1(MYH1) ELISA kit
E09M0413-192T	B-Gene	192 tests	EUR 1270
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Monkey Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Myosin 1(MYH1) ELISA kit
E09M0413-48	B-Gene	1 plate of 48 wells	EUR 520
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Monkey Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Myosin 1(MYH1) ELISA kit
E09M0413-96	B-Gene	1 plate of 96 wells	EUR 685
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Monkey Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Recombinant Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1)
4-RPA754Hu01	Cloud-Clone	EUR 481.70 EUR 232.00 EUR 1531.36 EUR 577.12 EUR 1054.24 EUR 385.00 EUR 3678.40	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Uniprot ID: P12882 Buffer composition: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. Form: Freeze-dried powder Predicted Molecular Mass (KD): 27.0kDa Isoelectric Point: 9.6
Description: Recombinant Human Myosin Heavy Chain 1, Skeletal Muscle, Adult expressed in: E.coli

Recombinant Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1)
4-RPA754Mu01	Cloud-Clone	EUR 503.20 EUR 238.00 EUR 1612.00 EUR 604.00 EUR 1108.00 EUR 400.00 EUR 3880.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Uniprot ID: Q5SX40 Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. Form: Freeze-dried powder Predicted Molecular Mass (KD): 37.5kDa Isoelectric Point: 5.6
Description: Recombinant Mouse Myosin Heavy Chain 1, Skeletal Muscle, Adult expressed in: E.coli

Guinea pig Myosin 1(MYH1) ELISA kit
E05M0413-192T	B-Gene	192 tests	EUR 1270
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Guinea pig Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Myosin 1(MYH1) ELISA kit
E05M0413-48	B-Gene	1 plate of 48 wells	EUR 520
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Guinea pig Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Myosin 1(MYH1) ELISA kit
E05M0413-96	B-Gene	1 plate of 96 wells	EUR 685
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Guinea pig Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Myosin heavy polypeptide 1 (MYH1) polyclonal antibody
ABP-PAB-01616	Allele Biotech	100 ug	Ask for price
Product line: Miscellaneous Brand:

ELISA kit for Bovine Myosin-1 (MYH1)
KTE10236-48T	Abbkine	48T	EUR 354
Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosi Show more
Description: Quantitative sandwich ELISA for measuring Bovine Myosin-1 (MYH1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Bovine Myosin-1 (MYH1)
KTE10236-5platesof96wells	Abbkine	5 plates of 96 wells	EUR 2252
Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosi Show more
Description: Quantitative sandwich ELISA for measuring Bovine Myosin-1 (MYH1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Bovine Myosin-1 (MYH1)
KTE10236-96T	Abbkine	96T	EUR 572
Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosi Show more
Description: Quantitative sandwich ELISA for measuring Bovine Myosin-1 (MYH1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Canine Myosin-1 (MYH1)
KTE20052-48T	Abbkine	48T	EUR 354
Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosi Show more
Description: Quantitative sandwich ELISA for measuring Canine Myosin-1 (MYH1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Canine Myosin-1 (MYH1)
KTE20052-5platesof96wells	Abbkine	5 plates of 96 wells	EUR 2252
Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosi Show more
Description: Quantitative sandwich ELISA for measuring Canine Myosin-1 (MYH1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Canine Myosin-1 (MYH1)
KTE20052-96T	Abbkine	96T	EUR 572
Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosi Show more
Description: Quantitative sandwich ELISA for measuring Canine Myosin-1 (MYH1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) Protein
20-abx168162	Abbexa	EUR 676.00 EUR 272.00 EUR 2068.00 EUR 801.00 EUR 481.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-7 working days.

Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) Antibody
20-abx005265	Abbexa	EUR 411.00 EUR 592.00 EUR 182.00 EUR 314.00	100 ul 200 ul 20 ul 50 ul
Shipped within 5-10 working days.

Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) Antibody
20-abx177671	Abbexa	EUR 1135.00 EUR 551.00	1 mg 200 ug
Please enquire.

Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) Antibody
20-abx212233	Abbexa	EUR 411.00 EUR 300.00	100 ul 50 ul
Shipped within 5-10 working days.

Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) Antibody
20-abx212641	Abbexa	EUR 411.00 EUR 300.00	100 ul 50 ul
Shipped within 5-10 working days.

Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) Antibody
20-abx104339	Abbexa	EUR 411.00 EUR 133.00 EUR 1135.00 EUR 551.00 EUR 314.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-7 working days.

Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) Antibody
20-abx128730	Abbexa	EUR 398.00 EUR 133.00 EUR 1107.00 EUR 537.00 EUR 314.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-7 working days.

Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) Antibody
20-abx173673	Abbexa	EUR 787.00 EUR 411.00	1 mg 200 ug
Please enquire.

Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) Antibody
20-abx320249	Abbexa	EUR 300.00 EUR 244.00	100 ul 50 ul
Shipped within 5-10 working days.

Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) Antibody
abx235469-100ug	Abbexa	100 ug	EUR 551
Shipped within 5-12 working days.

Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) Antibody
abx235470-100ug	Abbexa	100 ug	EUR 509
Shipped within 5-12 working days.

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit
abx053857-96tests	Abbexa	96 tests	EUR 754
Shipped within 5-12 working days.

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit
20-abx152417	Abbexa	EUR 6642.00 EUR 3542.00 EUR 825.00	10 × 96 tests 5 × 96 tests 96 tests
Shipped within 5-7 working days.

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit
abx253693-96tests	Abbexa	96 tests	EUR 754
Shipped within 5-12 working days.

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit
abx570236-96tests	Abbexa	96 tests	EUR 668
Shipped within 5-12 working days.

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) CLIA Kit
20-abx491987	Abbexa	EUR 7973.00 EUR 4246.00 EUR 981.00	10 × 96 tests 5 × 96 tests 96 tests
Please enquire.

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult(MYH1)ELISA Kit
QY-E03032	Qayee Biotechnology	96T	EUR 361

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit
SEA754Hu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 4273.35
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) were tested on 3 different plates, 8 replicates in each plate CV(%) = SD/meanX100 Intra-Assay: CV<10 Show more
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit
SEA754Hu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 439.57
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) were tested on 3 different plates, 8 replicates in each plate CV(%) = SD/meanX100 Intra-Assay: CV<10 Show more
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit
SEA754Hu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 585.1
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) were tested on 3 different plates, 8 replicates in each plate CV(%) = SD/meanX100 Intra-Assay: CV<10 Show more
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit
SEA754Hu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 2332.95
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) were tested on 3 different plates, 8 replicates in each plate CV(%) = SD/meanX100 Intra-Assay: CV<10 Show more
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit
4-SEA754Hu	Cloud-Clone	EUR 4324.00 EUR 2283.00 EUR 586.00	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Known also as Myosin Heavy Chain 1, Skeletal Muscle, Adult elisa. Alternative names of the recognized antigen: MYHSA1 MYHa MyHC-2X/D Myosin-1 Myosin Heavy Chain IIx/d
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) in samples from Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) Protein
20-abx068140	Abbexa	EUR 704.00 EUR 286.00 EUR 2165.00 EUR 829.00 EUR 495.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-7 working days.

Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) Antibody (FITC)
20-abx271252	Abbexa	EUR 453.00 EUR 244.00 EUR 1316.00 EUR 634.00 EUR 342.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-15 working days.

Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) Antibody (Biotin)
20-abx271518	Abbexa	EUR 425.00 EUR 230.00 EUR 1219.00 EUR 592.00 EUR 328.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-15 working days.

Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) Antibody Pair
20-abx370650	Abbexa	EUR 1622.00 EUR 1038.00	10 × 96 tests 5 × 96 tests
Shipped within 5-15 working days.

Recombinant Treponema pallidum p15 (partial)
7-07552	CHI Scientific	100µg	Ask for price

Recombinant Treponema pallidum p15 (partial)
7-07553	CHI Scientific	500µg	Ask for price

Recombinant Treponema pallidum p15 (partial)
7-07554	CHI Scientific	1000µg	Ask for price

Recombinant Treponema pallidum p17 (Partial)
7-07564	CHI Scientific	100µg	Ask for price

Recombinant Treponema pallidum p17 (Partial)
7-07565	CHI Scientific	500µg	Ask for price

Recombinant Treponema pallidum p17 (Partial)
7-07566	CHI Scientific	1000µg	Ask for price

Recombinant Treponema pallidum p47 (Partial)
7-07576	CHI Scientific	100µg	Ask for price

Recombinant Treponema pallidum p47 (Partial)
7-07577	CHI Scientific	500µg	Ask for price

Recombinant Treponema pallidum p47 (Partial)
7-07578	CHI Scientific	1000µg	Ask for price

Recombinant Treponema pallidum TmpA (Partial)
7-07579	CHI Scientific	100µg	Ask for price

Recombinant Treponema pallidum TmpA (Partial)
7-07580	CHI Scientific	500µg	Ask for price

Recombinant Treponema pallidum TmpA (Partial)
7-07581	CHI Scientific	1000µg	Ask for price

MYL5 Myosin Light Chain 5 (1-173 a.a.) Human Recombinant Protein
PROTQ02045-1	BosterBio	Regular: 10ug	EUR 317
Description: _x000D_ MYL5 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 197 amino acids (1-173 a.a.) and having a molecular mass of 22.1kDa.;MYL5 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

ELISA kit for Human MYH1 (Myosin Heavy Chain 1, Skeletal Muscle, Adult)
E-EL-H1430	Elabscience Biotech	1 plate of 96 wells	EUR 534
Gentaur's MYH1 ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MYH1. Standards or samples are added to the micro ELISA plate wells and combined with th Show more
Description: A sandwich ELISA kit for quantitative measurement of Human MYH1 (Myosin Heavy Chain 1, Skeletal Muscle, Adult) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human MYH1 (Myosin Heavy Chain 1, Skeletal Muscle, Adult)
ELK2107	ELK Biotech	1 plate of 96 wells	EUR 432
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated ant Show more
Description: A sandwich ELISA kit for detection of Myosin Heavy Chain 1, Skeletal Muscle, Adult from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) Polyclonal Antibody (Human, Pig)
4-PAA754Hu01	Cloud-Clone	EUR 232.00 EUR 2285.00 EUR 574.00 EUR 289.00 EUR 208.00	100ul 10ml 1ml 200ul 20ul
Sequence of the immunogen: MYH1 (Tyr585~Ala794) Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human, Pig Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1)

Mouse Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit
20-abx154425	Abbexa	EUR 6642.00 EUR 3542.00 EUR 825.00	10 × 96 tests 5 × 96 tests 96 tests
Shipped within 5-7 working days.

Pig Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit
abx360530-96tests	Abbexa	96 tests	EUR 825
Shipped within 5-12 working days.

Monkey Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit
abx359028-96tests	Abbexa	96 tests	EUR 825
Shipped within 5-12 working days.

Chicken Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit
abx355635-96tests	Abbexa	96 tests	EUR 825
Shipped within 5-12 working days.

Rabbit Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit
abx363586-96tests	Abbexa	96 tests	EUR 825
Shipped within 5-12 working days.

Sheep Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit
abx364394-96tests	Abbexa	96 tests	EUR 926
Shipped within 5-12 working days.

Высокоэффективная жидкостная хроматография в частично денатурирующих условиях ( dHPLC ) – это быстрый и экономичный метод скрининга молекулярных дефектов: четыре новые мутации, обнаруженные при Х-сцепленной хронической гранулематозной болезни.

 Внедрение точных методов в рутинной диагностике хронической гранулематозной болезни (ХГБ), которые ускоряют скрининг молекулярных дефектов, может иметь решающее значение для быстрого предположения прогноза пациента. В этом исследовании сравнивалась эффективность анализа однонитевого конформационного полиморфизма (SSCP) и высокоэффективной жидкостной хроматографии в частично денатурирующих условиях (dHPLC) для скрининга мутаций у пациентов с CGD. Мы отобрали 10 пациентов мужского пола с ХГБ, в анамнезе которых были тяжелые рецидивирующие инфекции и нарушение функции респираторного взрыва. Образцы гДНК, мРНК и кДНК получали стандартными методами. Экзоны CYBB амплифицировали с помощью ПЦР и проверяли с помощью SSCP или dHPLC. Аномальные фрагменты ДНК секвенировали, чтобы выявить природу мутаций.

Методы SSCP и dHPLC показали аномалии ДНК соответственно в 55% и 100% случаев. Секвенирование образцов аномальной ДНК подтвердило мутации во всех случаях. Были идентифицированы четыре новые мутации в CYBB, которые были обнаружены только при скрининге dHPLC (c.904 insC, c.141 + 5 g> t, c.553 T> C и c.665 A> T) . Эта работа подчеркивает актуальность dHPLC, чувствительного, быстрого, надежного и экономичного метода скрининга мутаций при CGD, который в сочетании с функциональными анализами, оценивающими респираторный взрыв фагоцитов, будет способствовать ускорению окончательной диагностики X-сцепленного CGD, непосредственно лечение, генетическое консультирование и четкое предположение о прогнозе. Эта стратегия особенно подходит для развивающихся стран.

Использование  DHPLC  (денатурирующая высокоэффективная жидкостная хроматография) при скрининге II уровня гена CFTR в пренатальной диагностике.

 ЦЕЛЬ

Цель исследования – оценить роль денатурирующей высокоэффективной жидкостной хроматографии (DHPLC) во втором уровне скрининга гена регулятора трансмембранной проводимости при муковисцидозе (CFTR).

МЕТОДЫ

9-месячное проспективное исследование, проведенное в период с июня 2008 г. по март 2009 г. в Медицинском центре Artemisia Fetal Medical Heart, включало 3829 образцов околоплодных вод у женщин, перенесших амниоцентез в середине триместра. Генетический диагноз CF был основан на исследовании основных мутаций CFTR. ген на ДНК плода, выделенный из амниоцитов (скрининг первого уровня) с использованием различных коммерческих диагностических систем. Скрининг второго уровня с использованием DHPLC на амниотической жидкости и образце крови пары был предложен в случае гетерозиготных на первом уровне плода.

ПОЛУЧЕННЫЕ РЕЗУЛЬТАТЫ

Из 3829 плодов 134 оказались положительными, 129 – гетерозиготными и 5 – пораженными . Из 129 пар после соответствующего генетического консультирования 53 запросили скрининг второго уровня. При использовании DHPLC 44 пары оказались отрицательными, а в девяти парах были идентифицированы девять редких мутаций.

ВЫВОДЫ

Скрининг первого уровня может быть полезен для выявления до 75% мутаций МВ . Скрининг второго уровня позволяет выявить еще 10% мутантных аллелей. Было обнаружено, что DHPLC является надежным и специфическим методом быстрой идентификации редких мутаций CFTR, которые не были обнаружены при первоначальном скрининге первого уровня.

 Эффективность dHPLC для полуавтоматического анализа кДНК-AFLP и сбора фрагментов в модели гена устойчивости к парше яблони.

Анализ кДНК-AFLP для профилирования транскриптов был успешно применен для изучения многих биологических систем растений, особенно взаимодействий между растениями и микробами. Однако, как сообщается , разделение фрагментов кДНК-AFLP с помощью гель-электрофореза является трудоемким с ограниченным потенциалом автоматизации, а сбор дифференциальных полос для идентификации генов является еще более трудоемким.

В этой работе мы представляем использование dHPLC (денатурирующая высокоэффективная жидкостная хроматография) и автоматизированный сбор фрагментов ДНК с использованием системы WAVE (®) для анализа и восстановления фрагментов кДНК-AFLP .

Этот метод успешно применяется к взаимодействию Malus-Venturia inaequalis , что позволяет собрать 66 различных фрагментов, полученных из транскриптов для генов яблока, предположительно участвующих в защитной реакции, активируемой геном устойчивости к HcrVf2.

Результаты, подтвержденные количественной ОТ-ПЦР в реальном времени, согласуются с исследуемым взаимодействием растения и патогена, и это дополнительно подтверждает пригодность dHPLC для профилирования транскриптов кДНК-AFLP . Обсуждаются особенности и преимущества этого нового подхода, демонстрирующие, что это почти полностью автоматизируемое и экономичное решение для обработки большого количества образцов и сбора дифференциальных генов, участвующих в других биологических процессах и немодельных растений.